Journal: Foods

Article Title: Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk

doi: 10.3390/foods11101450

Figure Lengend Snippet: Peptide BLG 40–60 showing the promote maturation ability of LP cells and directed differentiation of activated CD4 + T lymphocytes towards a Th1 phenotype, but not the proliferation, in the peptide–LP cell–MLN cell-co-culture assay. ( A ) The intensity of CFSE staining after stimulation. ( B ) The histogram results of CD80, CD86, TIM-4, CD28, and CD40. Each row corresponds to a different gate result, and each column corresponds to a different peptide treatment group. ( C ) Bar graphs showing the MFI levels summarized from ( B ). ( D ) Bar graphs showing the cytokines levels of IFN-γ, IL-4, IL-5, IL-13, IL-10, IL-17e, IL-12p70, and MIP-3α. ( E ) Bar graphs showing the IFN-γ/IL-4 ratio summarized from ( D ). The results are represented as the mean ± SD. (The data were calculated in three replicates for flow cytometry assay, and two replicates for cytokine level. The same letters indicate the lack of significance, as determined by two-way analysis of variance (ANOVA) with Duncan’s post-test ( p < 0.05).) LP cell, intestinal lamina propria cell. Th, T helper lymphocyte. MLN, mesenteric lymph node. CFSE, 5-(and 6)-carboxyfluorescein diacetate, succinimidyl ester. TIM-4, T cell immunoglobulin and mucin domain (TIM)-4. MFI, median fluorescence intensity. IFN-γ, interferon γ. IL, interleukin.

Article Snippet: MLN-derived CD4 + T cells were sorted by a CD4-positive sorting magnetic bead kit (anti-mouse CD4 magnetic particles-DM, 551539, BD Bioscience, Franklin Lakes, NJ, USA) according to the manufacturer’s instructions.

Techniques: Co-culture Assay, Staining, Flow Cytometry, Fluorescence

Journal: Foods

Article Title: Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk

doi: 10.3390/foods11101450

Figure Lengend Snippet: Peptides BLG 1–14 , BLG 40–60 , BLG 82–101 , and BLG 123–139 directed differentiation of activated MLN-CD4 + T lymphocytes towards a Th1 phenotype in the peptide–MLN cell-co-culture assay. ( A ) The histogram results of CD4 + IFN-γ + , CD4 + IL-4 + , CD4 + IL-17e + , and CD4 + CD28 + , and the contour plot of CD4 + CD25 + Foxp3 + after peptide stimulation. Each row corresponds to a different gate result, and each column corresponds to a different peptide treatment group. ( B ) Bar graphs showing the MFI levels summarized from ( A ). ( C ) Bar graphs showing the CD4 + IFN-γ + /CD4 + IL-4 + ratio summarized from ( B ). The results are represented as the mean ± SD ( n = 3, the data were calculated in three replicates, the same letters indicate the lack of significance, as determined by two-way analysis of variance (ANOVA) with Duncan’s post-test ( p < 0.05)). MLN, mesenteric lymph node. Th, T helper lymphocyte. MFI, median fluorescence intensity.

Article Snippet: MLN-derived CD4 + T cells were sorted by a CD4-positive sorting magnetic bead kit (anti-mouse CD4 magnetic particles-DM, 551539, BD Bioscience, Franklin Lakes, NJ, USA) according to the manufacturer’s instructions.

Techniques: Co-culture Assay, Fluorescence